The list by the All-China Federation of Industry and Commerce (ACFIC) came at the China Top 500 Private Enterprises Summit 2019 held in Xining, the capital of northwest China's Qinghai Province.
Huawei peaked the list with a revenue of 721.2 billion yuan (102 billion U.S. dollars) in 2018.
Top companies also include HNA Group, Suning, Amer International, Evergrande, JD.com, Country Garden, Hengli Group, Legend Holdings and Gome Holdings.
To be listed, an enterprise needed to have revenues exceeding 18.59 billion yuan last year.
A total of 20 of China's private firms were also on the list of the world's top 500 companies, three more than last year.
Latest comments