The Shenzhen Component Index closed 0.38 percent lower at 14,515.88 points.
The combined turnover of stocks covered by the two indices stood at 1.08 trillion yuan (about 168.9 billion U.S. dollars), up from 1.01 trillion yuan on the previous trading day.
Pharmaceutical companies were among those that gained the most, while liquor makers mostly lost.
The ChiNext Index, tracking China's NASDAQ-style board of growth enterprises, lost 0.3 percent to close at 3,399.66 points Wednesday.
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