Shares of Baidu and New Oriental Education & Technology Group lost 2.25 percent and 1.83 percent, respectively, the top two laggards in the top 10 stocks of the index.
Shares of NetEase increased 1.13 percent, leading the gainers.
U.S. stocks closed lower on Tuesday as investors monitored the latest development of trade issues between the United States and its major trading partners.
The Dow Jones Industrial Average decreased 280.23 points, or 1.01 percent, to 27,502.81. The S&P 500 fell 20.67 points, or 0.66 percent, to 3,093.20. The Nasdaq Composite Index was down 47.34 points, or 0.55 percent, to 8,520.64.
As of Monday, the S&P U.S. Listed China 50 index stood at 3,038.70, marking a 0.28-percent decrease for the month-to-date returns and a 32.62-percent gain for the year-to-date returns.
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